A Rearrangement of the Guanosine-Binding Site Establishes an Extended Network of Functional Interactions in the Tetrahymena Group I Ribozyme Active Site

Eitemau Tebyg

• Tightening of Active Site Interactions En-route to the Transition State Revealed by Single-Atom Substitution in the Guanosine-Binding Site of the Tetrahymena Group I Ribozyme
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• An active site rearrangement within the Tetrahymena group I ribozyme releases nonproductive interactions and allows formation of catalytic interactions
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• Structure and Function Converge To Identify a Hydrogen Bond in a Group I Ribozyme Active Site
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• Mutations at the guanosine-binding site of the Tetrahymena ribozyme also affect site-specific hydrolysis.
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• Guanosine binding to the Tetrahymena ribozyme: thermodynamic coupling with oligonucleotide binding.
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