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Pairwise selection assembly for sequence-independent construction of long-length DNA

The engineering of biological components has been facilitated by de novo synthesis of gene-length DNA. Biological engineering at the level of pathways and genomes, however, requires a scalable and cost-effective assembly of DNA molecules that are longer than ∼10 kb, and this remains a challenge. Her...

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Detalhes bibliográficos
Main Authors: Blake, William J., Chapman, Brad A., Zindal, Anuradha, Lee, Michael E., Lippow, Shaun M., Baynes, Brian M.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2860126/
https://ncbi.nlm.nih.gov/pubmed/20194119
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq123
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