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Creation of a type IIS restriction endonuclease with a long recognition sequence

Type IIS restriction endonucleases cleave DNA outside their recognition sequences, and are therefore particularly useful in the assembly of DNA from smaller fragments. A limitation of type IIS restriction endonucleases in assembly of long DNA sequences is the relative abundance of their target sites...

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Detalhes bibliográficos
Main Authors: Lippow, Shaun M., Aha, Patti M., Parker, Matthew H., Blake, William J., Baynes, Brian M., Lipovšek, Daša
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2009
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2685105/
https://ncbi.nlm.nih.gov/pubmed/19304757
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp182
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