Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly

In vitro recombination methods have enabled one-step construction of large DNA sequences from multiple parts. Although synthetic biological circuits can in principle be assembled in the same fashion, they typically contain repeated sequence elements such as standard promoters and terminators that in...

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Main Authors: Torella, Joseph P., Boehm, Christian R., Lienert, Florian, Chen, Jan-Hung, Way, Jeffrey C., Silver, Pamela A.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2014
Teme:
Synthetic Biology and Chemistry
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3874176/
https://ncbi.nlm.nih.gov/pubmed/24078086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt860
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