Unique nucleotide sequence (UNS)-guided assembly of repetitive DNA parts for synthetic biology applications

Recombination-based DNA construction methods, such as Gibson assembly, have made it possible to easily and simultaneously assemble multiple DNA parts and hold promise for the development and optimization of metabolic pathways and functional genetic circuits. Over time, however, these pathways and ci...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Nat Protoc
Main Authors: Torella, Joseph P., Lienert, Florian, Boehm, Christian R., Chen, Jan-Hung, Way, Jeffrey C., Silver, Pamela A.
Format: Artigo
Jezik:Inglês
Izdano: 2014
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4899833/
https://ncbi.nlm.nih.gov/pubmed/25101822
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.145
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