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Unique nucleotide sequence (UNS)-guided assembly of repetitive DNA parts for synthetic biology applications
Recombination-based DNA construction methods, such as Gibson assembly, have made it possible to easily and simultaneously assemble multiple DNA parts and hold promise for the development and optimization of metabolic pathways and functional genetic circuits. Over time, however, these pathways and ci...
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| Publicado no: | Nat Protoc |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4899833/ https://ncbi.nlm.nih.gov/pubmed/25101822 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.145 |
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