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Assays for the Preferential Binding of Human Topoisomerase I to Supercoiled DNA
To assay the preferential binding of eukaryotic type IB topoisomerases to supercoiled DNA, two methods are described that make use of a catalytically inactive mutant form of the enzyme. In the gel shift assay, the preference for binding to supercoiled plasmid DNA is detected in the presence of linea...
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| Main Authors: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2009
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2850600/ https://ncbi.nlm.nih.gov/pubmed/19763941 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60761-340-4_5 |
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