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Negative Effects of Sample Pooling on PCR-Based Estimates of Soil Microbial Richness and Community Structure

In this study, we examined the effect of various pooling strategies on the characterization of soil microbial community composition and phylotype richness estimates. Automated ribosomal intergenic spacer analysis (ARISA) profiles were determined from soil samples that were (i) unpooled (extracted an...

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Autors principals: Manter, Daniel K., Weir, Tiffany L., Vivanco, Jorge M.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2849261/
https://ncbi.nlm.nih.gov/pubmed/20139317
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03017-09
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