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Negative Effects of Sample Pooling on PCR-Based Estimates of Soil Microbial Richness and Community Structure

In this study, we examined the effect of various pooling strategies on the characterization of soil microbial community composition and phylotype richness estimates. Automated ribosomal intergenic spacer analysis (ARISA) profiles were determined from soil samples that were (i) unpooled (extracted an...

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Hlavní autoři: Manter, Daniel K., Weir, Tiffany L., Vivanco, Jorge M.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2849261/
https://ncbi.nlm.nih.gov/pubmed/20139317
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03017-09
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