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Negative Effects of Sample Pooling on PCR-Based Estimates of Soil Microbial Richness and Community Structure
In this study, we examined the effect of various pooling strategies on the characterization of soil microbial community composition and phylotype richness estimates. Automated ribosomal intergenic spacer analysis (ARISA) profiles were determined from soil samples that were (i) unpooled (extracted an...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2849261/ https://ncbi.nlm.nih.gov/pubmed/20139317 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03017-09 |
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