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Negative Effects of Sample Pooling on PCR-Based Estimates of Soil Microbial Richness and Community Structure

In this study, we examined the effect of various pooling strategies on the characterization of soil microbial community composition and phylotype richness estimates. Automated ribosomal intergenic spacer analysis (ARISA) profiles were determined from soil samples that were (i) unpooled (extracted an...

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Detalhes bibliográficos
Main Authors: Manter, Daniel K., Weir, Tiffany L., Vivanco, Jorge M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2849261/
https://ncbi.nlm.nih.gov/pubmed/20139317
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03017-09
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