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Removal of deaminated cytosines and detection of in vivo methylation in ancient DNA

DNA sequences determined from ancient organisms have high error rates, primarily due to uracil bases created by cytosine deamination. We use synthetic oligonucleotides, as well as DNA extracted from mammoth and Neandertal remains, to show that treatment with uracil–DNA–glycosylase and endonuclease V...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Briggs, Adrian W., Stenzel, Udo, Meyer, Matthias, Krause, Johannes, Kircher, Martin, Pääbo, Svante
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 2010
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC2847228/
https://ncbi.nlm.nih.gov/pubmed/20028723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1163
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