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Removal of deaminated cytosines and detection of in vivo methylation in ancient DNA
DNA sequences determined from ancient organisms have high error rates, primarily due to uracil bases created by cytosine deamination. We use synthetic oligonucleotides, as well as DNA extracted from mammoth and Neandertal remains, to show that treatment with uracil–DNA–glycosylase and endonuclease V...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2847228/ https://ncbi.nlm.nih.gov/pubmed/20028723 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1163 |
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