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PCS-based structure determination of protein–protein complexes
A simple and fast nuclear magnetic resonance method for docking proteins using pseudo-contact shift (PCS) and (1)H(N)/(15)N chemical shift perturbation is presented. PCS is induced by a paramagnetic lanthanide ion that is attached to a target protein using a lanthanide binding peptide tag anchored a...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Springer Netherlands
2010
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2844537/ https://ncbi.nlm.nih.gov/pubmed/20300805 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-010-9401-4 |
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