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Purification of Transmembrane Proteins from Saccharomyces cerevisiae for X-ray Crystallography

To enhance the quantity and quality of eukaryotic transmembrane proteins (TMPs) available for structure determination by x-ray crystallography, we have optimized protocols for purification of TMPs expressed in the yeast Saccharomyces cerevisiae. We focused on a set of the highest-expressing endogeno...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Clark, Kathleen M., Fedoriw, Nadia, Robinson, Katrina, Connelly, Sara M., Randles, Joan, Malkowski, Michael G., DeTitta, George T., Dumont, Mark E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2843815/
https://ncbi.nlm.nih.gov/pubmed/20045057
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2009.12.012
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