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Picomole-scale characterization of protein stability and function by quantitative cysteine reactivity

The Gibbs free energy difference between native and unfolded states (“stability”) is one of the fundamental characteristics of a protein. By exploiting the thermodynamic linkage between ligand binding and stability, interactions of a protein with small molecules, nucleic acids, or other proteins can...

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Detalhes bibliográficos
Main Authors: Isom, Daniel G., Vardy, Eyal, Oas, Terrence G., Hellinga, Homme W.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2841886/
https://ncbi.nlm.nih.gov/pubmed/20194783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0910421107
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