A Flow Cytometry-Based FRET Assay to Identify and Analyse Protein-Protein Interactions in Living Cells

BACKGROUND: Försters resonance energy transfer (FRET) microscopy is widely used for the analysis of protein interactions in intact cells. However, FRET microscopy is technically challenging and does not allow assessing interactions in large cell numbers. To overcome these limitations we developed a...

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Autors principals: Banning, Carina, Votteler, Jörg, Hoffmann, Dirk, Koppensteiner, Herwig, Warmer, Martin, Reimer, Rudolph, Kirchhoff, Frank, Schubert, Ulrich, Hauber, Joachim, Schindler, Michael
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2010
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Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2825263/
https://ncbi.nlm.nih.gov/pubmed/20179761
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0009344
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