A Flow Cytometry-Based FRET Assay to Identify and Analyse Protein-Protein Interactions in Living Cells

BACKGROUND: Försters resonance energy transfer (FRET) microscopy is widely used for the analysis of protein interactions in intact cells. However, FRET microscopy is technically challenging and does not allow assessing interactions in large cell numbers. To overcome these limitations we developed a...

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Main Authors: Banning, Carina, Votteler, Jörg, Hoffmann, Dirk, Koppensteiner, Herwig, Warmer, Martin, Reimer, Rudolph, Kirchhoff, Frank, Schubert, Ulrich, Hauber, Joachim, Schindler, Michael
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2010
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2825263/
https://ncbi.nlm.nih.gov/pubmed/20179761
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0009344
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