A novel FRET pair for detection of parallel DNA triplexes by the LightCycler

BACKGROUND: Melting temperature of DNA structures can be determined on the LightCycler using quenching of FAM. This method is very suitable for pH independent melting point (Tm) determination performed at basic or neutral pH, as a high throughput alternative to UV absorbance measurements. At acidic...

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Autori principali: Schneider, Uffe V, Severinsen, Jette K, Géci, Imrich, Okkels, Limei M, Jøhnk, Nina, Mikkelsen, Nikolaj D, Klinge, Teena, Pedersen, Erik B, Westh, Henrik, Lisby, Gorm
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2010
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Methodology Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2823659/
https://ncbi.nlm.nih.gov/pubmed/20102641
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-4
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