A novel FRET pair for detection of parallel DNA triplexes by the LightCycler

BACKGROUND: Melting temperature of DNA structures can be determined on the LightCycler using quenching of FAM. This method is very suitable for pH independent melting point (Tm) determination performed at basic or neutral pH, as a high throughput alternative to UV absorbance measurements. At acidic...

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Sonraí Bibleagrafaíochta
Main Authors: Schneider, Uffe V, Severinsen, Jette K, Géci, Imrich, Okkels, Limei M, Jøhnk, Nina, Mikkelsen, Nikolaj D, Klinge, Teena, Pedersen, Erik B, Westh, Henrik, Lisby, Gorm
Formáid: Artigo
Teanga:Inglês
Foilsithe: BioMed Central 2010
Ábhair:
Methodology Article
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2823659/
https://ncbi.nlm.nih.gov/pubmed/20102641
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-4
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