Improved Efficiency and Robustness in qPCR and Multiplex End-Point PCR by Twisted Intercalating Nucleic Acid Modified Primers

We introduce quantitative polymerase chain reaction (qPCR) primers and multiplex end-point PCR primers modified by the addition of a single ortho-Twisted Intercalating Nucleic Acid (o-TINA) molecule at the 5′-end. In qPCR, the 5′-o-TINA modified primers allow for a qPCR efficiency of 100% at signifi...

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Detalhes bibliográficos
Main Authors: Schneider, Uffe Vest, Mikkelsen, Nikolaj Dam, Lindqvist, Anja, Okkels, Limei Meng, Jøhnk, Nina, Lisby, Gorm
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3368873/
https://ncbi.nlm.nih.gov/pubmed/22701644
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0038451
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