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Nonaplex PCR using Cliffhanger primers to identify diarrhoeagenic Escherichia coli from crude lysates of human faecal samples

Sensitive, probe-based detection of multiple DNA targets is limited by the competitive reannealing of the antiparallel duplex DNA helix with the complementary DNA strand. To address this, we developed Cliffhanger primers, which create single-stranded DNA overhangs on PCR amplicons while simultaneous...

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Detaylı Bibliyografya
Yayımlandı:PLoS One
Asıl Yazarlar: Schneider, Uffe Vest, Mikkelsen, Nikolaj Dam, Scheutz, Flemming, Friis-Møller, Alice, Lisby, Gorm
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Public Library of Science 2018
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC6019694/
https://ncbi.nlm.nih.gov/pubmed/29944710
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0199766
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