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Incorporation of the fluorescent ribonucleotide analogue tCTP by T7 RNA polymerase
Fluorescent RNA is an important analytical tool in medical diagnostics, RNA cytochemistry and RNA aptamer development. We have synthesized the fluorescent ribonucleotide analogue 1,3-diaza-2-oxophenothiazine-ribose-5′-triphosphate (tCTP) and tested it as substrate for T7 RNA polymerase in transcript...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2819109/ https://ncbi.nlm.nih.gov/pubmed/20067253 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac902456n |
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