Quantitative analysis of ribosome–mRNA complexes at different translation stages

Inhibition of primer extension by ribosome–mRNA complexes (toeprinting) is a proven and powerful technique for studying mechanisms of mRNA translation. Here we have assayed an advanced toeprinting approach that employs fluorescently labeled DNA primers, followed by capillary electrophoresis utilizin...

Full description

Saved in:
Bibliographic Details
Main Authors: Shirokikh, Nikolay E., Alkalaeva, Elena Z., Vassilenko, Konstantin S., Afonina, Zhanna A., Alekhina, Olga M., Kisselev, Lev L., Spirin, Alexander S.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2010
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2817456/
https://ncbi.nlm.nih.gov/pubmed/19910372
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1025
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items