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Quantitative analysis of ribosome–mRNA complexes at different translation stages

Inhibition of primer extension by ribosome–mRNA complexes (toeprinting) is a proven and powerful technique for studying mechanisms of mRNA translation. Here we have assayed an advanced toeprinting approach that employs fluorescently labeled DNA primers, followed by capillary electrophoresis utilizin...

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Bibliografische gegevens
Hoofdauteurs: Shirokikh, Nikolay E., Alkalaeva, Elena Z., Vassilenko, Konstantin S., Afonina, Zhanna A., Alekhina, Olga M., Kisselev, Lev L., Spirin, Alexander S.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2010
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2817456/
https://ncbi.nlm.nih.gov/pubmed/19910372
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1025
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