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Quantitative analysis of ribosome–mRNA complexes at different translation stages
Inhibition of primer extension by ribosome–mRNA complexes (toeprinting) is a proven and powerful technique for studying mechanisms of mRNA translation. Here we have assayed an advanced toeprinting approach that employs fluorescently labeled DNA primers, followed by capillary electrophoresis utilizin...
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Main Authors: | , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Oxford University Press
2010
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2817456/ https://ncbi.nlm.nih.gov/pubmed/19910372 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1025 |
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