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A single-label phenylpyrrolocytidine provides a molecular beacon-like response reporting HIV-1 RT RNase H activity

6-Phenylpyrrolocytidine (PhpC), a structurally conservative and highly fluorescent cytidine analog, was incorporated into oligoribonucleotides. The PhpC-containing RNA formed native-like duplex structures with complementary DNA or RNA. The PhpC-modification was found to act as a sensitive reporter g...

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Detalhes bibliográficos
Main Authors: Wahba, Alexander S., Esmaeili, Abbasali, Damha, Masad J., Hudson, Robert H. E.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2817455/
https://ncbi.nlm.nih.gov/pubmed/19933258
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1022
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