Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of the putative SAICAR synthetase (PH0239) from Pyrococcus horikoshii OT3

The study of proteins involved in de novo biosynthesis of purine nucleotides is central in the development of antibiotics and anticancer drugs. In view of this, a protein from the hyperthermophile Pyrococcus horikoshii OT3 was isolated, purified and crystallized using the microbatch method. Its prim...

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Bibliografische gegevens
Hoofdauteurs: Manjunath, Kavyashree, Jeyakanthan, Jeyaraman, Nakagawa, Noriko, Shinkai, Akeo, Yoshimura, Masato, Kuramitsu, Seiki, Yokoyama, Shigeyuki, Sekar, Kanagaraj
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: International Union of Crystallography 2010
Onderwerpen:
Crystallization Communications
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2815687/
https://ncbi.nlm.nih.gov/pubmed/20124717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109052026
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