Overexpression, purification, crystallization and preliminary crystallographic studies of a hyperthermophilic adenylosuccinate synthetase from Pyrococcus horikoshii OT3

Adenylosuccinate synthetase (AdSS) is a ubiquitous enzyme that catalyzes the first committed step in the conversion of inosine monophosphate (IMP) to adenosine monophosphate (AMP) in the purine-biosynthetic pathway. Although AdSS from the vast majority of organisms is 430–457 amino acids in length,...

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Dettagli Bibliografici
Autori principali: Wang, Xiaoying, Akasaka, Ryogo, Takemoto, Chie, Morita, Satoshi, Yamaguchi, Machiko, Terada, Takaho, Shirozu, Mikako, Yokoyama, Shigeyuki, Chen, Shilin, Si, Shuyi, Xie, Yong
Natura: Artigo
Lingua:Inglês
Pubblicazione: International Union of Crystallography 2011
Soggetti:
Crystallization Communications
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3232137/
https://ncbi.nlm.nih.gov/pubmed/22139164
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430911104108X
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