Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of the putative SAICAR synthetase (PH0239) from Pyrococcus horikoshii OT3

The study of proteins involved in de novo biosynthesis of purine nucleotides is central in the development of antibiotics and anticancer drugs. In view of this, a protein from the hyperthermophile Pyrococcus horikoshii OT3 was isolated, purified and crystallized using the microbatch method. Its prim...

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Detalhes bibliográficos
Main Authors: Manjunath, Kavyashree, Jeyakanthan, Jeyaraman, Nakagawa, Noriko, Shinkai, Akeo, Yoshimura, Masato, Kuramitsu, Seiki, Yokoyama, Shigeyuki, Sekar, Kanagaraj
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2815687/
https://ncbi.nlm.nih.gov/pubmed/20124717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109052026
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