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In vitro footprinting of promoter regions within supercoiled plasmid DNA

Polypurine/polypyrimidine (pPU/pPY) tracts, which exist in the promoter regions of many growth-related genes, have been proposed to be very dynamic in their conformation. In this chapter, we describe a detailed protocol for DNase I and S1 nuclease footprinting experiments with supercoiled plasmid DN...

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Bibliografische gegevens
Hoofdauteur: Sun, Daekyu
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2010
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2807360/
https://ncbi.nlm.nih.gov/pubmed/19997887
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-418-0_14
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