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In vitro footprinting of promoter regions within supercoiled plasmid DNA
Polypurine/polypyrimidine (pPU/pPY) tracts, which exist in the promoter regions of many growth-related genes, have been proposed to be very dynamic in their conformation. In this chapter, we describe a detailed protocol for DNase I and S1 nuclease footprinting experiments with supercoiled plasmid DN...
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2807360/ https://ncbi.nlm.nih.gov/pubmed/19997887 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-418-0_14 |
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