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Rapid "footprinting" on supercoiled DNA.
A DNase protection technique is described and applied to the interaction of three lac control proteins with supercoiled lac DNA. The technique uses end-labeled oligonucleotide primers to probe specific DNA regions as an alternative to protocols requiring restriction endonuclease cleavage or blotting...
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Autor principal: | |
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Formato: | Artigo |
Lenguaje: | Inglês |
Publicado: |
1985
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Materias: | |
Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC397717/ https://ncbi.nlm.nih.gov/pubmed/3889902 |
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