Rapid "footprinting" on supercoiled DNA.
A DNase protection technique is described and applied to the interaction of three lac control proteins with supercoiled lac DNA. The technique uses end-labeled oligonucleotide primers to probe specific DNA regions as an alternative to protocols requiring restriction endonuclease cleavage or blotting...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Hovedforfatter: | |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1985
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397717/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3889902/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.10.3078 |
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