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HOW ACCURATELY CAN A SINGLE MOLECULE BE LOCALIZED WHEN IMAGED THROUGH AN OPTICAL MICROSCOPE?

We present a simple analytical expression for the fundamental limit to the accuracy with which the location of a single molecule can be determined that is imaged through an optical microscope. This expression depends on the optical properties of the microscope and the photophysical properties of the...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Ram, Sripad, Ward, E. Sally, Ober, Raimund J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2004
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2800313/
https://ncbi.nlm.nih.gov/pubmed/20046822
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1109/ISBI.2004.1398731
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