Site-directed spin labeling of a genetically encoded unnatural amino acid

The traditional site-directed spin labeling (SDSL) method, which utilizes cysteine residues and sulfhydryl-reactive nitroxide reagents, can be challenging for proteins that contain functionally important native cysteine residues or disulfide bonds. To make SDSL amenable to any protein, we introduce...

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Autori principali: Fleissner, Mark R., Brustad, Eric M., Kálai, Tamás, Altenbach, Christian, Cascio, Duilio, Peters, Francis B., Hideg, Kálmán, Peuker, Sebastian, Schultz, Peter G., Hubbell, Wayne L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2009
Soggetti:
Biological Sciences
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2799802/
https://ncbi.nlm.nih.gov/pubmed/19995976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0912009106
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