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Display and selection of scFv antibodies on HEK-293T cells
We describe a human cell display strategy to isolate high affinity single chain antibody fragments (scFvs) specific for CD22 for the treatment of B-cell malignancies. Our strategy uses flow cytometry and human embryonic kidney 293T (HEK-293T) cells that are widely used for transient protein expressi...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2009
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2790380/ https://ncbi.nlm.nih.gov/pubmed/19554290 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-302-2_8 |
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