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Display and selection of scFv antibodies on HEK-293T cells

We describe a human cell display strategy to isolate high affinity single chain antibody fragments (scFvs) specific for CD22 for the treatment of B-cell malignancies. Our strategy uses flow cytometry and human embryonic kidney 293T (HEK-293T) cells that are widely used for transient protein expressi...

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Bibliografske podrobnosti
Main Authors: Ho, Mitchell, Pastan, Ira
Format: Artigo
Jezik:Inglês
Izdano: 2009
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2790380/
https://ncbi.nlm.nih.gov/pubmed/19554290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-302-2_8
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