Hydrophobic Photolabeling Studies Identify the Lipid-Protein Interface of the 5-HT(3A) Receptor

A HEK-293 cell line that stably expresses mouse 5-HT(3A)Rs containing a C-terminal extension that confers high affinity binding of alpha-bungarotoxin (αBgTx) was established (αBgTx-5-HT(3A)Rs) and used to purify αBgTx-5-HT(3A)Rs in a lipid environment for use in structural studies using photoaffinit...

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Hauptverfasser: Sanghvi, Mitesh, Hamouda, Ayman K., Davis, Margaret I., Morton, Russell A., Srivastava, Shouryadeep, Pandhare, Akash, Duddempudi, Phaneendra K., Machu, Tina K., Lovinger, David M., Cohen, Jonathan B., Blanton, Michael P.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2009
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2778300/
https://ncbi.nlm.nih.gov/pubmed/19715355
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi901208j
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