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Identifying the Lipid-Protein Interface of the α4β2 Neuronal Nicotinic Acetylcholine Receptor: Hydrophobic Photolabeling Studies with [(125)I]TID
Using an acetylcholine-derivatized affinity column, we have purified human α4β2 neuronal nicotinic acetylcholine receptors (nAChR) from a stably transfected HEK-293 cell line. Both the quantity and the quality of the purified receptor are suitable for applying biochemical methods to directly study t...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2007
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2597459/ https://ncbi.nlm.nih.gov/pubmed/17994769 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi701705r |
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