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Identifying the Lipid-Protein Interface of the α4β2 Neuronal Nicotinic Acetylcholine Receptor: Hydrophobic Photolabeling Studies with [(125)I]TID

Using an acetylcholine-derivatized affinity column, we have purified human α4β2 neuronal nicotinic acetylcholine receptors (nAChR) from a stably transfected HEK-293 cell line. Both the quantity and the quality of the purified receptor are suitable for applying biochemical methods to directly study t...

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Detalhes bibliográficos
Main Authors: Hamouda, Ayman K., Sanghvi, Mitesh, Chiara, David C., Cohen, Jonathan B., Blanton, Michael P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2597459/
https://ncbi.nlm.nih.gov/pubmed/17994769
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi701705r
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