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Prediction of hot spot residues at protein-protein interfaces by combining machine learning and energy-based methods

BACKGROUND: Alanine scanning mutagenesis is a powerful experimental methodology for investigating the structural and energetic characteristics of protein complexes. Individual amino-acids are systematically mutated to alanine and changes in free energy of binding (ΔΔG) measured. Several experiments...

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Detalhes bibliográficos
Main Authors: Lise, Stefano, Archambeau, Cedric, Pontil, Massimiliano, Jones, David T
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2777894/
https://ncbi.nlm.nih.gov/pubmed/19878545
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-10-365
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