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Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins
This protocol describes a detailed method to study the static and dynamic features of membrane proteins, as well as solvent accessibility, by utilizing the lactose permease of Escherichia coli (LacY) as a model. The method relies on the use of functional single-Cys mutants, an affinity tag and a Pho...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2775809/ https://ncbi.nlm.nih.gov/pubmed/17703213 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2007.275 |
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