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Green fluorescence induced by EF-hand assembly in a split GFP system
The affinity between the 1–157 and 158–238 fragments of green fluorescent protein (GFP) is too low for spontaneous in vivo reassembly of the protein upon co-expression of the two fragments. This prevents chromophore maturation and the cells lack GFP fluorescence. We have utilized the very high affin...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Wiley Subscription Services, Inc., A Wiley Company
2009
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2774432/ https://ncbi.nlm.nih.gov/pubmed/19472338 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.131 |
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