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Green fluorescence induced by EF-hand assembly in a split GFP system

The affinity between the 1–157 and 158–238 fragments of green fluorescent protein (GFP) is too low for spontaneous in vivo reassembly of the protein upon co-expression of the two fragments. This prevents chromophore maturation and the cells lack GFP fluorescence. We have utilized the very high affin...

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Autores principales: Lindman, Stina, Johansson, Ida, Thulin, Eva, Linse, Sara
Formato: Artigo
Lenguaje:Inglês
Publicado: Wiley Subscription Services, Inc., A Wiley Company 2009
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2774432/
https://ncbi.nlm.nih.gov/pubmed/19472338
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.131
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