Immunoassays with rolling circle DNA amplification: A versatile platform for ultrasensitive antigen detection

We describe an adaptation of the rolling circle amplification (RCA) reporter system for the detection of protein Ags, termed “immunoRCA.” In immunoRCA, an oligonucleotide primer is covalently attached to an Ab; thus, in the presence of circular DNA, DNA polymerase, and nucleotides, amplification res...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Schweitzer, Barry, Wiltshire, Steven, Lambert, Jeremy, O'Malley, Shawn, Kukanskis, Kari, Zhu, Zhengrong, Kingsmore, Stephen F., Lizardi, Paul M., Ward, David C.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2000
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC27732/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10954739/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.170237197
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