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Immunoassays with rolling circle DNA amplification: A versatile platform for ultrasensitive antigen detection

We describe an adaptation of the rolling circle amplification (RCA) reporter system for the detection of protein Ags, termed “immunoRCA.” In immunoRCA, an oligonucleotide primer is covalently attached to an Ab; thus, in the presence of circular DNA, DNA polymerase, and nucleotides, amplification res...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Schweitzer, Barry, Wiltshire, Steven, Lambert, Jeremy, O'Malley, Shawn, Kukanskis, Kari, Zhu, Zhengrong, Kingsmore, Stephen F., Lizardi, Paul M., Ward, David C.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2000
Schlagworte:
Biological Sciences
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC27732/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10954739/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.170237197
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