Immunoassays with rolling circle DNA amplification: A versatile platform for ultrasensitive antigen detection

We describe an adaptation of the rolling circle amplification (RCA) reporter system for the detection of protein Ags, termed “immunoRCA.” In immunoRCA, an oligonucleotide primer is covalently attached to an Ab; thus, in the presence of circular DNA, DNA polymerase, and nucleotides, amplification res...

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Главные авторы: Schweitzer, Barry, Wiltshire, Steven, Lambert, Jeremy, O'Malley, Shawn, Kukanskis, Kari, Zhu, Zhengrong, Kingsmore, Stephen F., Lizardi, Paul M., Ward, David C.
Формат: Artigo
Язык:Inglês
Опубликовано: The National Academy of Sciences 2000
Предметы:
Biological Sciences
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC27732/
https://ncbi.nlm.nih.gov/pubmed/10954739
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