Unbinding forces of single antibody-antigen complexes correlate with their thermal dissociation rates

Point mutants of three unrelated antifluorescein antibodies were constructed to obtain nine different single-chain Fv fragments, whose on-rates, off-rates, and equilibrium binding affinities were determined in solution. Additionally, activation energies for unbinding were estimated from the temperat...

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Bibliographic Details
Main Authors: Schwesinger, Falk, Ros, Robert, Strunz, Torsten, Anselmetti, Dario, Güntherodt, Hans-Joachim, Honegger, Annemarie, Jermutus, Lutz, Tiefenauer, Louis, Plückthun, Andreas
Format: Artigo
Language:Inglês
Published: The National Academy of Sciences 2000
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC27642/
https://ncbi.nlm.nih.gov/pubmed/10963664
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