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Unbinding forces of single antibody-antigen complexes correlate with their thermal dissociation rates
Point mutants of three unrelated antifluorescein antibodies were constructed to obtain nine different single-chain Fv fragments, whose on-rates, off-rates, and equilibrium binding affinities were determined in solution. Additionally, activation energies for unbinding were estimated from the temperat...
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| Główni autorzy: | , , , , , , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
The National Academy of Sciences
2000
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC27642/ https://ncbi.nlm.nih.gov/pubmed/10963664 |
| Etykiety: |
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