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Accelerating phage-display library selection by reversible and site-specific biotinylation
Immobilization of a target molecule to a solid support is an indispensable step in phage display library sorting. Here we describe an immobilization method that addresses shortcomings of existing strategies. Our method is based on the use of a polyhistidine-tagged (His-tagged) target molecule and (B...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2763796/ https://ncbi.nlm.nih.gov/pubmed/19737805 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzp053 |
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