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Engineering a G protein-coupled receptor for structural studies: Stabilization of the BLT1 receptor ground state
Structural characterization of membrane proteins is hampered by their instability in detergent solutions. We modified here a G protein-coupled receptor, the BLT1 receptor of leukotriene B(4), to stabilize it in vitro. For this, we introduced a metal-binding site connecting the third and sixth transm...
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| Main Authors: | , , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Wiley Subscription Services, Inc., A Wiley Company
2009
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2762585/ https://ncbi.nlm.nih.gov/pubmed/19309698 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.55 |
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