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Direct measurements of kinesin torsional properties reveal flexible domains and occasional stalk reversals during stepping

Kinesin is a homodimeric motor with two catalytic heads joined to a stalk via short neck linkers (NLs). We measured the torsional properties of single recombinant molecules by tracking the thermal angular motions of fluorescently labeled beads bound to the C terminus of the stalk. When kinesin heads...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Gutiérrez-Medina, Braulio, Fehr, Adrian N., Block, Steven M.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: National Academy of Sciences 2009
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC2761353/
https://ncbi.nlm.nih.gov/pubmed/19805111
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0907133106
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