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Direct measurements of kinesin torsional properties reveal flexible domains and occasional stalk reversals during stepping
Kinesin is a homodimeric motor with two catalytic heads joined to a stalk via short neck linkers (NLs). We measured the torsional properties of single recombinant molecules by tracking the thermal angular motions of fluorescently labeled beads bound to the C terminus of the stalk. When kinesin heads...
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| Principais autores: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2761353/ https://ncbi.nlm.nih.gov/pubmed/19805111 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0907133106 |
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